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KMID : 0352519930300010299
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Korea Univercity Medical Journal
1993 Volume.30 No. 1 p.299 ~ p.308
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Reperfusion Models of Rat Middle Cerebral Artery Occlusion: Extracranial and Transcranial Methods
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옥광휘
주정화/이훈갑/서중근/정여섭/박윤관/이기찬
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Abstract
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The author described a comparative study of 2 different experimental methods of reperfusion models in rat; 1) transcranial method (TC) and 2) extracranial method(EC). The transcranial method was performed by a direct approach to the middle
cerebral
artery (MCA) after temporozygomatic craniotomy in which a wire was hooked on the proximal portion of middle cerebral artery (MCA) and pulled it to make occlusion of MCA. For the extracranial method, a nylon surgical thread was inserted
intraluminally
along the internal carotid artery (ICA) to reach the anterior communicating artery. Recirculation of blood was accomplished by removal of the hook or of the thread after 4 hours occlusion of MCA. Two different models were subdeivided into 4
chronological groups: pre-reperfusion 30 minutes, 2 hours, and 48 hours atter reperfusion. The differences were evaluated with morphometric analysis after neutral red (NR) ro tetrazolium salt (TTC) staining.
From the evaluation of inital ischemic area, the extracranial method a larger infarct volume as compared to the transcranial method (P=0.013). Mean value(SD) of infarcted ara of EC and TC were 28.7%(4.4%) and 14.4%(8.1%) respectively. Recovery
from
the
initial ischemia after reperfusion was gradually achieved in both of two groups(p<0.05). The restoration of blood flow after reperfusion was more rapid in EC group and only 2 hours' reperfusion was enough to show no difference between two
methods.
In TC
group followed by 48 hours' reperfusion 40% of animals resulted in gross infarction of MCA territory as noted with TTC stain, most probably due to reperfusion failure. EC group showed only one such a case (12.5%). But this difference was not in
the
range of significance statistically. From a technical point of view, EC was more simple, needed less time since a craniotomy was not performed, and required no manipulation of the brain or any itracranial vessels. From these results the author
concluded that the extracranial method is a more reliable and technically more manageable one to be used as a reperfusion model for investigation the focal cerebral ischemia.
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KEYWORD
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